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If symptoms do not improve despite adequate B12 replacement, further investigation for other causes of nerve pain will be necessary

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The following solutions were mixed together: 100 µL of cells in TE buffer, 10 µL of herring sperm DNA (10 mg mL −1 ), 10 µL of SAT1 flipper disruption cassette (0.1 µg µL −1 ), and 5 µL of histamine solution (Qbiogene)